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aav ef1a flpo addgene  (Addgene inc)


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    Addgene inc aav ef1a flpo addgene
    Aav Ef1a Flpo Addgene, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 267 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/aav+flex+tdtomato/pAAV-FLEX-tdTomato+(Plasmid+%2328306)/pm41920739-171-80-81
    Average 96 stars, based on 267 article reviews
    aav ef1a flpo addgene - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Bioprocessing:

    Article Title: A melanopsin ganglion cell subtype forms a dorsal retinal mosaic projecting to the supraoptic nucleus
    Article Snippet: .. AAV-FLEX-tdTomato (Cat# 28306 AAV2 & PHPeB), AAV1-DF-ChR2-mCherry (Cat..# 18916), and AAV pCAG-FLEX-EGFP-WPRE (Cat.# 51502) AAV-CAG-DIO-ChR2(H134R)-EYFP (Cat #127090; AAV PHP.Eb) were purchased through Addgene. ..

    Article Title: The gut-brain vagal axis governs mesolimbic dopamine dynamics and reward events
    Article Snippet: .. Each virus was co-injected with an AAV-FLEX-tdTomato in a 1/10.000 ratio to visualize sparse neurons ( ex vivo electrophysiology and dendritic spine density). pAAV-FLEX-tdTomato was a gift from Edward Boyden (Addgene viral prep #28306-AAV9; http://n2t.net/addgene:28306 ; RRID:Addgene_28306). pAAV-hsyn-GRAB_DA2m was a gift from Yulong Li (Addgene viral prep #140553-AAV9; http://n2t.net/addgene:140553 ; RRID:Addgene_140553). pENN.AAV.CamKII.GCaMP6f.WPRE.SV40 was a gift from James M. Wilson (Addgene viral prep #100834-AAV9; http://n2t.net/addgene:100834 ; RRID:Addgene_100834). pAAV-hSyn-dLight1.2 was a gift from Lin Tian (Addgene viral prep #111068-AAV5; http://n2t.net/addgene:111068 ; RRID:Addgene_111068). .. For all surgical procedures, mice were rapidly anesthetized with isoflurane (3.5%, induction), injected (ip) with the analgesic buprenorphine (Buprecare, 0.3 mg/kg, Recipharm, Lancashire, UK) and ketoprofen (Ketofen, 10 mg/kg, France), and maintained under isoflurane anesthesia (1.5%) throughout the surgery.

    Article Title: A melanopsin ganglion cell subtype forms a dorsal retinal mosaic projecting to the supraoptic nucleus.
    Article Snippet: .. AAV-FLEX-tdTomato (Cat# 28306 AAV2 & PHPeB), AAV1-DFChR2-mCherry (Cat..# 18916), and AAV pCAG-FLEX-EGFP-WPRE (Cat.# 51502) AAV-CAG-DIO-ChR2(H134R)-EYFP (Cat #127090; AAV PHP.Eb) were purchased through Addgene. ..

    Article Title: Molecular Disambiguation of Heart Rate Control by the Nucleus Ambiguus
    Article Snippet: .. A pulled glass micropipette was used for stereotaxic injections of AAV-FLEX-tdTomato (Addgene plasmid # 28306; http://n2t.net/addgene:28306 ; RRID:Addgene_28306; a gift from Edward Boyden) or AAV-DIO-H2b-mCherry (Vigene Biosciences, 3.94 x 10 13 GC/m) using the following stereotaxic coordinates for the nucleus ambiguus: anterior/posterior -2.1, -2.4, -2.7 mm, lateral/medial +/- 1.3 mm, and dorsal/ventral - 5.8 mm, from lambda; and anterior/posterior -0.1 mm, lateral/medial +/- 1.3 mm, and dorsal/ventral -0.1, -1.3 mm, from the calamus scriptorus. ..

    Article Title: Activity of brain stem glucagon neurons are modulated by energy state and encode sex and frequency-dependent negative valence and anxiety
    Article Snippet: For viral injections, a glass capillary was inserted into a Nanoject III (Drummond Scientific; Broomall, PA) and attached to the stereotaxic frame. .. Either AAV-FLEX-tdTomato (200nl, Addgene – cat# 28306-AAV5, lot# v122060) or pAAV-Syn-Flex-ro[ChrimsonR-tdTomato] (200nl, Addgene – cat# 62723-AAV5, lot# v63071), were injected at +/- 0.4mm lateral, −0.3mm posterior, z: −4.0mm ventral of obex. ..

    Article Title: Pharmacological administration of FGF21 reverses obesity through a parabrachial-projecting neuron population in the hindbrain.
    Article Snippet: Images and movies of brain samples were captured with Imaris and edited in Shotcut. .. The following viral constructs were used in this study: AAV2/9CMVCreEGFP (University of Iowa viral vector core), AAV2/9CMVeGFP (University of Iowa viral vector core), AAV-hSyn-DIO-hM3D(Gq)-mCherry (Addgene # 44361-AAV2), AAV-hSyn-DIO-mCherry (Addgene # 50459-AAV2), AAV-hSyn-DIO-EGFP (Addgene # 50457-AAVrg), AAV-FLEX-tdTomato (Addgene #28306), AAV-EF1a-Flpo (Addgene # 55637-AAVrg), AAV-EF1a-fDIO-Cre (Addgene # 121675-AAV9). ..

    Article Title: Btbd11 supports cell-type-specific synaptic function
    Article Snippet: AAV.CMV.FII.eGFP-Cre.WPRE.SV40 (serotype 2/9) , Addgene , Cat# 105545-AAV9. .. AAV-FLEX-tdTomato , Addgene , Cat# 28306-AAV9. .. AAV-S5E2-dTom-nlsdTom (serotype 2/9) , Janelia Farm , N/A.

    Article Title: Hindbrain neurons that underlie water discrimination and consumption
    Article Snippet: Article Hindbrain neurons that underlie water discrimination and consumption

    Virus:

    Article Title: The gut-brain vagal axis governs mesolimbic dopamine dynamics and reward events
    Article Snippet: .. Each virus was co-injected with an AAV-FLEX-tdTomato in a 1/10.000 ratio to visualize sparse neurons ( ex vivo electrophysiology and dendritic spine density). pAAV-FLEX-tdTomato was a gift from Edward Boyden (Addgene viral prep #28306-AAV9; http://n2t.net/addgene:28306 ; RRID:Addgene_28306). pAAV-hsyn-GRAB_DA2m was a gift from Yulong Li (Addgene viral prep #140553-AAV9; http://n2t.net/addgene:140553 ; RRID:Addgene_140553). pENN.AAV.CamKII.GCaMP6f.WPRE.SV40 was a gift from James M. Wilson (Addgene viral prep #100834-AAV9; http://n2t.net/addgene:100834 ; RRID:Addgene_100834). pAAV-hSyn-dLight1.2 was a gift from Lin Tian (Addgene viral prep #111068-AAV5; http://n2t.net/addgene:111068 ; RRID:Addgene_111068). .. For all surgical procedures, mice were rapidly anesthetized with isoflurane (3.5%, induction), injected (ip) with the analgesic buprenorphine (Buprecare, 0.3 mg/kg, Recipharm, Lancashire, UK) and ketoprofen (Ketofen, 10 mg/kg, France), and maintained under isoflurane anesthesia (1.5%) throughout the surgery.

    Ex Vivo:

    Article Title: The gut-brain vagal axis governs mesolimbic dopamine dynamics and reward events
    Article Snippet: .. Each virus was co-injected with an AAV-FLEX-tdTomato in a 1/10.000 ratio to visualize sparse neurons ( ex vivo electrophysiology and dendritic spine density). pAAV-FLEX-tdTomato was a gift from Edward Boyden (Addgene viral prep #28306-AAV9; http://n2t.net/addgene:28306 ; RRID:Addgene_28306). pAAV-hsyn-GRAB_DA2m was a gift from Yulong Li (Addgene viral prep #140553-AAV9; http://n2t.net/addgene:140553 ; RRID:Addgene_140553). pENN.AAV.CamKII.GCaMP6f.WPRE.SV40 was a gift from James M. Wilson (Addgene viral prep #100834-AAV9; http://n2t.net/addgene:100834 ; RRID:Addgene_100834). pAAV-hSyn-dLight1.2 was a gift from Lin Tian (Addgene viral prep #111068-AAV5; http://n2t.net/addgene:111068 ; RRID:Addgene_111068). .. For all surgical procedures, mice were rapidly anesthetized with isoflurane (3.5%, induction), injected (ip) with the analgesic buprenorphine (Buprecare, 0.3 mg/kg, Recipharm, Lancashire, UK) and ketoprofen (Ketofen, 10 mg/kg, France), and maintained under isoflurane anesthesia (1.5%) throughout the surgery.

    Plasmid Preparation:

    Article Title: Molecular Disambiguation of Heart Rate Control by the Nucleus Ambiguus
    Article Snippet: .. A pulled glass micropipette was used for stereotaxic injections of AAV-FLEX-tdTomato (Addgene plasmid # 28306; http://n2t.net/addgene:28306 ; RRID:Addgene_28306; a gift from Edward Boyden) or AAV-DIO-H2b-mCherry (Vigene Biosciences, 3.94 x 10 13 GC/m) using the following stereotaxic coordinates for the nucleus ambiguus: anterior/posterior -2.1, -2.4, -2.7 mm, lateral/medial +/- 1.3 mm, and dorsal/ventral - 5.8 mm, from lambda; and anterior/posterior -0.1 mm, lateral/medial +/- 1.3 mm, and dorsal/ventral -0.1, -1.3 mm, from the calamus scriptorus. ..

    Injection:

    Article Title: Activity of brain stem glucagon neurons are modulated by energy state and encode sex and frequency-dependent negative valence and anxiety
    Article Snippet: For viral injections, a glass capillary was inserted into a Nanoject III (Drummond Scientific; Broomall, PA) and attached to the stereotaxic frame. .. Either AAV-FLEX-tdTomato (200nl, Addgene – cat# 28306-AAV5, lot# v122060) or pAAV-Syn-Flex-ro[ChrimsonR-tdTomato] (200nl, Addgene – cat# 62723-AAV5, lot# v63071), were injected at +/- 0.4mm lateral, −0.3mm posterior, z: −4.0mm ventral of obex. ..

    Construct:

    Article Title: Pharmacological administration of FGF21 reverses obesity through a parabrachial-projecting neuron population in the hindbrain.
    Article Snippet: Images and movies of brain samples were captured with Imaris and edited in Shotcut. .. The following viral constructs were used in this study: AAV2/9CMVCreEGFP (University of Iowa viral vector core), AAV2/9CMVeGFP (University of Iowa viral vector core), AAV-hSyn-DIO-hM3D(Gq)-mCherry (Addgene # 44361-AAV2), AAV-hSyn-DIO-mCherry (Addgene # 50459-AAV2), AAV-hSyn-DIO-EGFP (Addgene # 50457-AAVrg), AAV-FLEX-tdTomato (Addgene #28306), AAV-EF1a-Flpo (Addgene # 55637-AAVrg), AAV-EF1a-fDIO-Cre (Addgene # 121675-AAV9). ..



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